Genetic analysis of specific and non-specific immune response in Oreochomis niloticus L. - PhDData

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Genetic analysis of specific and non-specific immune response in Oreochomis niloticus L.

The thesis was published by Sarder, Md Rafiqul Islam, in September 2022, University of Stirling.

Abstract:

Gynogenesis or androgenesis were used to produce completely homozygous inbred tish, from which clonal lines were established by subsequent gynogenesis, androgenesis and sib mating. Homozygosity of the inbred lines was verified using multilocus DNA fingerprinting and isozyme locus ADA*, which showed all gynogenetic offspring to have only maternal inheritance. DNA fingerprints of meiotic and mitotic gynogens showed bands consistent with those of their mother but not of their father. Inbred clones also showed identical banding patterns with their mitotic mother and not their father. The outbred clones, in contrast, shared bands with both parents according to Mendelian inheritance.
Sex ratios of both meiotic and mitotic gynogenetics were analysed and a significantly higher number of females (P



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