This thesis investigates the relationship between plant parasitic nematodes and endophytic bacteria at the rhizospheric ecosystem of Robusta coffee in Vietnam. From there, it further investigates and evaluates the potential effects of endophytic bacteria, in particular Streptomyces spp., on phytopathogens and plant growth. The interdisciplinary studies as executed in this thesis are based on several approaches. Firstly, the relationships between nematodes – plants – bacteria are assessed by environmental surveys combined with statistical methods. Secondly, in vivo and in vitro screening assays are utilised to choose the bacterial candidates with desired antimicrobial and anti-nematode profiles. Thirdly, in silico genome mining is used to discover potential genetic clusters encoding for the aforementioned antagonizing properties. Finally, a greenhouse experiment was conducted to evaluate the potential application of the bacterial candidate(s). Overall, this work demonstrates the enormous potential of a Streptomyces strain, as bioactive agent which may be perfectly suitable for combating phytopathogens in coffee plants.