With the rapid development of nanotechnology in the twenty-first century, many solutions for ideally suited material with special characteristics have been proposed. Miniaturization of laboratory instruments achieved by Nanotechnology allows efficient use of reagents, faster reaction speed, and precise parameter measurements. Separation and filtration of biological particles is an important step in qualitative and quantitative analysis experiments in the biochemistry and biomedical industries. The membrane microfiltration technique can be used to improve the effectiveness of microfluidic devices used in filtration and separation processes at the micro- and nano-level. Therefore , it is obvious that developing an efficient and commercially available membrane technology for the separation/filtration of micro-/nano-particles based on the phenomenon of surface acoustics is crucial. Aluminum oxide nanomembrane research is still in its early stage, so it’s important to develop methods to expand their applications and increase knowledge of their production processes. The research and development of nanoporous alumina membranes for filtration/separation processes is presented in this dissertation, including the fabrication process, characterization, property characterization, and modulation of surface acoustic waves on the surface of a nanoporous AAO membrane using a PZT transducer. Furthermore, the advantages of using a nanoporous AAO membrane for gas separation are discussed.