Satureja montana L. is widely used as culinary herb and in traditional medicine for the treatment or relief of various common health symptoms in many parts of the world. However, numerous studies have shown that it has pharmacological potential to be used as antibacterial drug, antidiabetic, anti-HIV and antiproliferative drug. Its pharmacological effects can be attributed to the presence of phytochemicals like flavonoids, sterols, tannins and essential oils. Phenolic compounds are mostly responsible for the anti-oxidative activity of Satureja montana L. In this research, the aim was to determine the influence of Satureja montana L. extract on glycemia, lipid status and biochemical parameters, indicators of liver and kidney function in the serum of healthy and diabetic rats and rats fed with high fat diet, as well as to examine it influence on the parameters of oxidative stress in animals exposed to the toxic dose of paracetamol. For determination of hypoglycemic and antihyperlipidemic effect of Satureja montana L., Wistar laboratory rats were divided into 8 groups, in groups used to determine hypoglycemic potential diabetes was induced by streptozotocin and after that, animals were treated for 28 days per os with saline solution, metformin 100 mg/kg, Satureja montana extract 500 mg/kg and their combination. For examination of hypolipidemic effect animals were treated with high fat diet and saline solution, simvastatin 10 mg/kg, Satureja montana L. extract 500 mg/kg and their combination. For determination of antioxidant potential of Satureja montana L. animals were divided into four groups and winter savory extract and saline solution were given them orally for 7 days. After that, rats were exposed to the toxic dose of paracetamol. Biochemical parameters, the lipids concentration and parameters of the kidney and liver function, were determined in animal serum. Ex vivo analyses included the determination of lipid peroxidation activity and the activity of antioxidant protection enzymes in liver homogenates of the test animals, after administration of a toxic dose of paracetamol. In vitro analyses were performed with extracts obtained with different solvents. Antioxidant activity tests as well as total phenolic and flavonoid content, were determined spectrophotometrically. In diabetic animals, 28 days treatment with Satureja montana L. extract brought to statistically significant decrease of glucose comparing to the control. Statistically significantly lower TG values were observed in the group that received Satureja montana L. extract compared to the control diabetic group, at the end of the study. On the other parameter of lipid status, the examined extract did not show significance effects. In animals fed with food enriched with fats, application of Satureja montana L. extract brought to statistically significant decrease of LDL cholesterol, relative to the control group fed with food enriched with fats. Concentration of parameters of the kidney and liver function: AST, urea, creatinine, and uric acid were significantly lower in the group treated with Satureja montana L. extract in comparison to the control group fed with food enriched with fats. Pre-treatment of animals with herbal extracts before application of the toxic dose of paracetamol significantly decreased alkaline phosphatase activity, concentration of direct bilirubin and creatinine in the serum relative to the group treated with saline solution and single dose of paracetamol. The activity of lipid peroxidation was significantly higher in animals that were treated with saline solution compared with Satureja montana L. extract, before the toxic doses of paracetamol. In addition, in animals pretreated with Satureja montana L. extract, the number of inflammatory cells is decreased along with the activity of CYP2E1 enzymes. The results of these studies showed hypoglycemic potential of Satureja montana L. extract in animals with induced diabetes. Pretreatment with Satureja montana L. extract prior to the administration of the toxic dose of paracetamol has shown potential of hepatoprotective, anti-inflammatory and antioxidative effect. Also, PCA analyses showed that the extraction mediums had a large influence on the antioxidative effect.