Characterisation of extracellular products produced by Mycobacterium spp. and their effects on the fish immune system - PhDData

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Characterisation of extracellular products produced by Mycobacterium spp. and their effects on the fish immune system

The thesis was published by Chen, Shih-Chu, in September 2022, University of Stirling.

Abstract:

Mycobacterium spp. isolated from food and ornamental fish in Thailand (strains TB 1, TB40, TB267, TB268), and the type strains Mycobacterium marinum (NCIMB 1298), Mycobacterium fortuitum (NCIMB 1294), and Mycobacterium chelonae (NClMB 1474) were cultured in Long’s medium, Eagle’s minimum essential medium, Sauton’s medium and modified Sauton’s medium. The latter enabled excellent growth and production of extracellular products (ECP) from TB40, TB267, TB268 and M marinum in particular, whereas growth and production of ECP for all strains was limited in Long’s medium. SDS-PAGE protein profiles of ECPs from 14 day culture supernatants showed major bands at 65, and



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